An ecdysteroid-regulated 16-kDa protein homologue is involved in the immune response of the crayfish Procambarus clarkii (2023)

partial fragment

Animal and sampling

In June, he collected river crab samples (weight: 6-7 g) in Hopei Market (Hefei, China).They were kept at room temperature during the 12 -hour photo and fed small vertebrates for 10 days.Collect its muscles, liver, G, blood cells and intestines for extracting RNA and protein.For immune challenges, 10 μg of fat polysaccharide (LPS) was injected into each river crab, each river crab was collected in 0, 12, 48, and 48 rivers, and collected and 72 hours.Three hours

Sequence analysispersonal computer-E16

One sentence 453PC-E16ofP. ClarkiiIt is characterized, encoding 150 amino acid wastes, with a calculated molecular weight of approx. 16 kda.personal computerThe sequence-E16 contains signal peptides (1-17 AA) and lipid recognition domain (ML) related to MD-2.Three -dimensional structurepersonal computer-E16 mainly includes β-folding.Multiple sequence comparison analysis indicatespersonal computer-E16 shared 38.03%–41.96% identity with insect ESR16 proteins (Fig. 1A). Phylogenetic relationship among 19


A protein regulated by ecdysteroids is characterized by a conserved ML domainP. Clarkii。ML domains are present in several proteins from different species and function in various biological processes through interactions with lipids [30,31].ESR16ofM. SextayesA. Khamenei,,,,PC-E16It is also widely expressed in different organizations.personal computer-e16 shows greater similarity to invertebrate-regulated proteinspersonal computer-E16 is the same

Credit authorization contribution statement

: Ao lille l iU :Writing -original draft, method, research.A child zhang:Research, data management.Z and Chen:Project management and verification.yuan yuan Fe of:Methodology, Visualization.DAO Jun Wang:Software, computer management.B aojian Z Tiger:Surveillance, acquisition funds, writing: review and editing.

Competitive interests declaration

The authors have no conflicts of interest to declare.


This work is supported byNatural Science Foundation of Anhui Province[Authorization Number1908085MC77],Anhui Agricultural University Innovation Research Group Foundation[Authorization NumberAnrc2019032] andNatural Science Foundation of Colleges and Universities in Anhui Province[Authorization Number2022 UH 050923]。

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